Bioactivity of Benzo[a]pyrene against the expression of CD4+TNFα, CD8+ IFNŸ, and CD4+IL1+ in mice (Mus Musculus L) after paramyxovirus measles vaccine administration

Kumpulan	: Jurnal Ilmiah (Non-Kategori) [Inggris]
Edisi/Volume	: ,
Pengarang	: Suwoyo Suwoyo1, Muhaimin Rifa’i2*, Widodo2, Muhammad Sasmito Djati2
Klasifikasi/Subjek	: ,
Penerbitan	: Brawijaya University, Malang: 2019.
Bahasa	: Inggris
PENYIMPANAN
Lokasi	: PUSAT-25-A-
Jumlah	: 1

Abstraksi

ABSTRACT Background: Benzo[a]pyrene is one of the external factors that reduce immunity. The disease arises by the accumulation of benzo[a]pyrene in DNA, which forms benzo[a]pyrene and aryl hydrocarbon receptor (AhR bonds). The bond of benzo[a] pyrene and AhR could affect the differentiation of immune cells then induce an immunosuppressive effect. Objective: This research aims to identify the bioactivity of benzo[a]pyrene toward the expression of CD4+ tumor necrosis factor (TNF)α, CD8+ IFNŸ, and CD4+ interleukin (IL)1+ in mice treated with a paramyxovirus vaccine. Materials and Methods: This research was conducted using a split-plot design. The bioactivity analysis of benzo[a]pyrene toward immune cells expression in mice (Mus musculus L) was analyzed by administration of benzo[a]pyrene at 20 mg/kg BW through intramuscular injection 2 times/week for 4 weeks. The expression of CD4+ TNF-α+, CD8+ IFN-Ÿ+, and CD4+IL-1+ was analyzed using flow cytometry. Results: Group with vaccine and benzo[a]pyrene exposure at week 4 showed that TNFα was repressed while IFNŸ was elevated. Furthermore, the modest regulatory function of CD4+ was activated. Conclusion: The exposure of benzo[a]pyrene to the mice (M. musculus L) with dose 20 mg/kg BW for 2 times/weeks exhibited the immune cell repression on CD4+TNFα+ and immune cell activation on CD8+IFNŸ+, while CD4+IL-1+ at week 4.

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